RNA-Seq of E14 MGE, Prdm16 WT vs cKO

This study aimed to understand the role of the transcriptional regulator Prdm16 in the development of cortical interneurons in the mouse. Prdm16 was knocked out in cells derived from the medial ganglionic eminence (MGE) by using an Nkx2.1-Cre driver line in combination with a line carrying floxed Prdm16 alleles and with a Cre-dependent tdTomato reporter line (Ai14). The sequencing data compares the gene expression profiles of dissected MGEs at embryonic day 14 (E14), a stage when cortical interneurons are being generated from MGE progenitors. Overall design: The MGEs of 3 pools of 3 embryos each were compared between control (labeled as "WT", containing Cre and Ai14 alleles) and mutant (labeled as "KO", homozygous for the floxed version of the Prdm16 allele) samples.