McrA gene sequencing using two different primer pairs

The main goal of this work was to evaluate the sensitivity of two different primers for the purpose of sequencing the functional mcrA gene. In this work, the results of NGS sequencing are compared using ML primer (ML) and mlas-mod primer (MLAS). Both of these primers are recommended for sequencing the gene for the methyl coenzyme reductase of methanogenic archaea. This enzyme is not present in non-methane producing microorganisms and is therefore typical and highly selective for especialy for methanogens.