Ascl2-dependent cell differentiation drives regeneration of abated intestinal stem cells

Functions of Ascl2 in colonic stem cell maintenance and regeneration. We used RNA-sequencing to profile gene expression in regenerating crypt cells and Ascl2-deficient colonic stem cells, and ASCL2 ChIP-seq to identify transcriptional target genes. Overall design: RNA-seq and ASCL2 ChIP-seq analyses of isolated mouse colonic stem cells. RNA-seq analyses of Ascl2-deficient mouse colonic stem cells. Single Cell RNA-seq analysis of regenerating colonic crypt cells. Transcript dynamics of colonic epithelial development.