Neuromodulatory co-expression in cardiac vagal motor neurons of the Dorsal Motor Nucleus of the Vagus [10x scRNA]

The activity of the cardiac vagal innervation is well known to be crucial to the maintenance of cardiac health, and to protect and recover the heart from injury. Only recently has this role been shown to depend on the activity of the underappreciated Dorsal Motor Nucleus of the Vagus (DMV). By combining neural tracing, transcriptomics, and 3D mapping in male and female Sprague Dawley rats we characterize cardiac-specific neuronal phenotypes in DMV. We find that the DMV cardiac-projecting neurons differentially express PACAP, CART, and synucleins, as well as evidence that they participate in neuromodulatory co-expression involving catecholamines. The significance of these findings is enhanced by previous knowledge of the role of PACAP at the heart and of the other neuromodulators in peripheral vagal targets. We generated a single cell suspension from the dorsal medulla including the DMV by a dissociation protocol for adult rat brain (Wang et al. 2021), and viability was determined by calcein AM stain. The 4 samples included pools of 4 left and right dorsal brainstem from 12 week old male Sprague Dawley rats. We obtained sc-RNAseq data using 10x Genomics Chromium assay, available as a TJU core facility service.