Sizes and morphology of coccoliths from DSDP Site 72-516

Long-term alkenone-based pCO2 records are widely applied in paleoclimate evaluations. These pCO2 estimates are based on records of the carbon isotope fractionation that occurs during marine haptophyte photosynthesis (epsilon p37:2). In addition to the concentration of aqueous CO2 (CO2(aq)) the magnitude of epsilon p37:2 is also influenced by algal growth rates and cell geometry. To date, the influence of haptophyte cell geometry on the expression of ancient epsilon p37:2 values has received little attention. This study evaluates changes in cell geometry of ancient alkenone-producing algae at Deep See Drilling Project Site 516 in the southwest Atlantic Ocean by analyzing individual coccolith dimensions, which are proportional to algal cell volume and surface area. We show that during part of the early Miocene, mean cell sizes of alkenone-producing algae were smaller relative to modern Emiliania huxleyi. Cell size variations coincide with significant changes in epsilon p37:2, with a distinct 6 per mil decrease in epsilon p37:2 at ∼20.3 Ma associated with a 27% increase in haptophyte cell sizes. These changes in cell size impact epsilon p37:2-based interpretations of growth rate variation and CO2(aq) estimates for this southwest Atlantic site. After correcting for cell geometry, CO2(aq) estimates at Site 516 are consistent with those reported from other oligotrophic sites during this time, resulting in overall low atmospheric pCO2 estimates (<350 ppmv) for the early Miocene.