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Identification of the cellular component interacted with F. nucleatum.

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We incubated F. nucleatum lysates with biotinylated proteins from KRAS p.G12D, KRAS p.G13D and KRAS WT cells respectively. Subsequent western blot analysis showed that three candidate proteins from F. nucleatum might interact with KRAS p.G12D cells. The bands of interest were then submitted for mass spectrometry (MS) protein identification, which reported three proteins.
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2024-01-25
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