mRNA levels in hypothalamus of rainbow trout incubated in vitro for 1 h at 15°C in modified Hanks’ medium containing 2 mM D-glucose alone (control) or containing 1, 10, or 100 µM oleic acid or octanoic acid.

Each value is the mean of 5 independent experiments carried out with pools of hypothalami from 3–4 different fish. Data is expressed as fold-induction (+, increase; −, decrease) with respect to the control group (expression results were normalized by β-actin mRNA levels, mRNA levels-no variation). *, significantly different (P