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NGS result of FrCas9 in rice cells
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2024-09-18
相关数据集
A Type II-B Cas9 nuclease with no detectable off-targets and reduced chromosomal translocations in vivo.
Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as components of CRISPR-Cas9 genome editing and engineering platforms. However, they exhibit promiscuous nuclease activity that
NIAID Data Ecosystem60
CRISPR-Cas9 knockout screens using the miRNA-targeting lentiG-miR library
The data includes NGS data analyses from CRISPR-Cas9 knockout screens for 47 human cancer cell lines, performed with PoolQ (v2.2.0). This output includes raw read counts of sgRNAs and corresponding qu
Figshare2024-04-25 更新40
The safety of highly stable Cas9 in mice
Twenty-four male C57BL/6 mice (5 weeks old) were randomly divided into three groups (NC, Cas9, and HSCas9 groups). NC group were hydrodynamically injected with the plasmid pBB4.5-1.3HBV, PX458-Cas9, a
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gene editing in rice by CRISPR/Cpf1
To demonstrate Cpf1 can be engineered to modify PAM recognition, broadening the targeting range in rice.
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Genome editing in rice mediated by miniature size Cas nuclease SpCas12f. Sukegawa et al.
Electrophoresis results for HMA analysis (Figure 2A) and Sanger sequencing chromatogram results for T1 plants (Figure 4B). The HMA results (Figure 2A) show 20 samples of each target. Sanger sequencing
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