Describing molecular coupling of the estrogen receptor and coregulators and its crosstalk with retinoic acid receptors in breast cancer

Purpose: We exploit transcriptomic data to shed light on the functional consequences of ER-RAR crosstalk mediated by RIP140 and LCoR. We compare ligand-stimulated conditions with and without knocked down of RIP140 and LCoR. We also assess the additive versus synergistic nature of global transcriptional changes by comparing separate 17-ß estradiol (E2) and retinoic acid (RA) induced transcriptomes with the transcriptome induced by simultaneous E2 and RA stimulation. Overall design: 16 experimental conditions available, 1 duplicate in 1 cell line (MELN)