Characterization of Uba2 sumoylation.
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(A) Effect of uba2-K229R mutations on the ability of Aos1-Uba2 in catalyzing sumoylation reaction. (B) Effect of uba2-K229R and uba2-3KR on Uba2 sumoylation in vivo. Whole cell lysates from the indicated yeast strains were subjected to Western blot analysis. (C) Effect of uba2-K229R and uba2-3KR mutations on cell growth. The mec1Δ mutant is used as a positive control for sensitivity to hydroxyurea (HU), camptothecin (CPT) and UV.
创建时间:
2016-02-23



