Comparison of STEEL siRNA-treated and control siRNA-treated human endothelial cells. Comparison of STEEL siRNA-treated and control siRNA-treated human endothelial cells

To determine the role of STEEL in endothelial cell (EC) gene regulation, gene expression analysis was conducted on control and STEEL siRNA-treated human dermal microvascular endothelial cells (HMVECs) and human umbilical vein endothelial cells (HUVECs). A total of 225 protein-coding genes were downregulated and 80 were upregulated when both EC types were grouped for analysis. In HMVEC alone, 544 protein-coding genes were downregulated and 218 were upregulated. In HUVEC alone, 177 protein-coding genes were downregulated and 125 were upregulated. Prominently, STEEL siRNA depletion results in the downregulation of two notable protein-coding genes, eNOS and KLF2, which are modulated in ECs subjected to continuous laminar shear stress. Overall design: HUVECs and HMVECs were treated with control siRNA or STEEL siRNA for 48 hours. Subsequently, these samples were subjected to gene expression analysis using a custom agilent microarray that recognizes 30215 protein-coding transcripts and 33045 lncRNAs.