Table_1_A non-targeted metabolomics analysis identifies wound-induced oxylipins in Physcomitrium patens.xlsx

Plant oxylipins are a class of lipid-derived signaling molecules being involved in the regulation of various biotic and abiotic stress responses. A major class of oxylipins are the circular derivatives to which 12-oxo-phytodienoic acid (OPDA) and its metabolite jasmonic acid (JA) belong. While OPDA and its shorter chain homologue dinor-OPDA (dnOPDA) seem to be ubiquitously found in land plants ranging from bryophytes to angiosperms, the occurrence of JA and its derivatives is still under discussion. The bryophyte Physcomitrium patens has received increased scientific interest as a non-vascular plant model organism over the last decade. Therefore, we followed the metabolism upon wounding by metabolite fingerprinting with the aim to identify jasmonates as well as novel oxylipins in P. patens. A non-targeted metabolomics approach was used to reconstruct the metabolic pathways for the synthesis of oxylipins, derived from roughanic, linoleic, α-linolenic, and arachidonic acid in wild type, the oxylipin-deficient mutants of Ppaos1 and Ppaos2, the mutants of Ppdes being deficient in all fatty acids harboring a Δ6-double bond and the C20-fatty acid-deficient mutants of Ppelo. Beside of OPDA, iso-OPDA, dnOPDA, and iso-dnOPDA, three additional C18-compounds and a metabolite being isobaric to JA were identified to accumulate after wounding. These findings can now serve as foundation for future research in determining, which compound(s) will serve as native ligand(s) for the oxylipin-receptor COI1 in P. patens.

植物氧脂类是一种由脂质衍生而来的信号分子，其在调节多种生物和非生物胁迫响应中发挥着重要作用。氧脂类的主要一类是环状衍生物，其中12-氧代植二烯酸（OPDA）及其代谢物茉莉酸（JA）属于此类。尽管OPDA及其较短的链状同源物二去甲基-OPDA（dnOPDA）似乎在从苔藓植物到被子植物的陆生植物中普遍存在，但JA及其衍生物的存在仍存在争议。在过去十年中，苔藓植物Physcomitrium patens作为非维管植物模式生物体，受到了越来越多的科学关注。因此，我们通过代谢指纹分析跟踪了伤口愈合过程中的代谢途径，旨在识别P. patens中的茉莉酸类物质以及新型氧脂类。采用非靶向代谢组学方法，重建了从粗糙酸、亚油酸、α-亚麻酸和花生四烯酸合成的氧脂类的代谢途径，这些途径来源于野生型、氧脂类缺乏型突变体Ppaos1和Ppaos2、缺乏所有含Δ6-双键的脂肪酸的Ppdes突变体以及C20-脂肪酸缺乏型Ppelo突变体。除了OPDA、异-OPDA、dnOPDA和异-dnOPDA之外，还鉴定出三种额外的C18化合物以及一种与JA同位素的代谢物，这些物质在受伤后积累。这些发现现在可作为未来研究的基础，以确定哪些化合物将作为P. patens中氧脂类受体COI1的天然配体（s）。