Tup1 stabilizes promoter nucleosome positioning and occupancy at transcriptionally plastic genes.

Chromatin mapping by MNase-Seq, using a novel technical approach (Matched MNase Digestions)that enables high-throughput sequencing technology to identify specific and accurate differences between broadly similar chromatin states. In this case we compare chromatin structures in the presence and absence of the well-characterized repressor protein in S. cerevisiae, Tup1.