Development and validation of methods that enable high-quality droplet digital PCR and hematological profiling data from microvolume blood samples: Supplementary tables

Aim: Mouse models have been crucial to preclinical studies in the increasingly relevant fields of cell and
gene therapy. However, only small quantities of mouse blood can be collected without producing adverse
physiological effects that compromise data integrity. Results: To address this limitation, two combined
methods were developed to create detailed droplet digital PCR (ddPCR) and hematological profiles using
only ∼20 μl of mouse blood. The validation of these methods, which can serve as a foundation for a
standardized regulatory pipeline for ddPCR, is discussed. Even when using small amounts of input, this
ddPCR protocol is accurate, precise, selective, specific, stable and robust. Conclusion: These techniques
enable more frequent sample collection for higher-resolution pharmacokinetic data that meets or exceeds
quality standards.