TABLE 1 in A new species of Sisubiotus (Tardigrada: Macrobiotidae) from northern Sweden with a key to the genus

TABLE 1. PCR protocols and primers for the amplification of the DNA fragments sequenced in this study. MarkerPrimerSequence 5’ - 3’SourceCOILCO1490GGT CAA CAA ATC ATA AAG ATA TTG GFolmer et al., 1994HCO2190TAA ACT TCA GGG TGA CCA AAA AAT CAFolmer et al., 1994Protocol: 5 min @ 94°C; (45s@94°C, 45s@50°C, 60s@72°C) x 40; 10 min @ 72°C18S3FGTT CGA TTC CGG AGA GGG AGiribet et al., 199682RTGA TCC TTC TGC AGG TTC ACC TACSands et al., 2008Protocol: 5 min @ 94°C; (45s@94°C, 45s@50°C, 90s@72°C) x 35; 10 min @ 72°C28SEutar_FACC CGC TGA ACT TAA GCA TATGąsiorek et al., 2018R0990CCT TGG TCC GTG TTT CAA GACMironov et al., 2012Protocol: 5 min @ 94°C; (30s@94°C, 90s@53°C, 60s@72°C) x 35; 10 min @ 72°C