Examination of RNA editing levels on miR-379-410 cluster in Brain mice

We performed RNA sequencing of pri-miRNAs and mature miRNAs of the miR-379-410 cluster in juvenile mouse brain to determine differences in editing levels at these two stages of miRNA maturation. We selected seven pri-miRNAs previously shown to be subjected to adenosine-to-inosine RNA editing, and used gene-specific primers to make double-stranded cDNA of the pri-miRNAs to be sequenced. Editing levels of the sites in the pri-miRNAs were compared to editing levels of the corresponding sites in mature miRNAs after performing small RNA sequencing of the same samples (more detail here?). We found that editing levels differed in pri- and mature miRNAs in a miRNA-specific manner; where the majority of sites had lower editing levels in the mature miRNA. In contrast, editing of miR-376b-3p was higher in the mature miRNA than in the pre-miRNA.