Kif5C associated RNAs from mouse hippocampus

To identify Kif5C transported RNAs, we carried out co-immunoprecipitation of KIf5C-RNA protein complexes from mouse hippocampus. RNAseq analysis reveal ~600 RNAs differentially enriched in the Kif5C complexes suggesting that these are are likely transported to the dendrites by Kif5C. These RNAs code for regulators of structural re-arrangements, translation and synaptic remodeling. Co-immunoprecpitation (Co-IP) of Kif5C from mouse hippocampus using an antibody that can succesfully immunoprecipitate Kif5C from mouse hippocampus. Co-IP using IgG Ab was used as control.For each Co-IP, we used hippocampi from two adult mice. For the RNAseq analysis, we pooled 6 different Co-IP samples into two samples. Two samples corresponding to Kif5C Co-IP and two for the control Co-IP. Following IP, RNAs were isolated using Trizol method and submitted to Scripps Genomics Core for Processing.