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In vivo development of rat 2-cell embryos produced from oocytes that were injected with heads from spermatozoa heat-dried and stored for various periods in nitrogen gas and induced activation by a single direct current pulse (1 kV/cm) for 100 µsec+DMAP.

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a–cValues with different superscripts within column differ significantly (P<0.05). 1Control (unheated) was not activated by a single direct current pulse (1 kV/cm) for 100 µsec+DMAP. 2One young died 7 days after delivery. 3The young died on the next day of delivery.

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2013-11-04
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