RNA-seq of S. cerevisiae (BY4741) treated with Lanosterol against untreated controls in the presence and absence of alpha-factor

We have performed paired-end RNA sequencing on S. cerevisiae (BY4741). Lanosterol (LST) was predicted as a potential modulator of the Ste2 (GPCR)-mediated pheromone-induced cell death using Gcoupler. The cells were grown at 30°C at 200 r.p.m. in YPD medium in biological duplicates in the presence or absence of lanosterol (LST) at a final concentration of 1 µM. Post-metabolite treatment, the cells were either treated or untreated with the a-factor at a final concentration of 30 µM for 2 hours at desired growth conditions. Post these steps, the cells were harvested, and RNA was isolated, and sequenced.