Lipid content of fractionated lipoproteins.
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ApoE-free (E–) lipoproteins were incubated on a nutating shaker for 1 h at room temperature with apoE3 (E–/E3) or E4 (E–/E4). The ratio of apoEs to E– lipoprotein was 1 to 10 (protein to protein). The lipids were then fractionated by FPLC. Total cholesterol (TC) and free cholesterol (FC) in apoE-free and apoE-enriched lipoproteins were measured by colorimetry. Cholesterol ester was calculated as the difference between TC and FC.
创建时间：
2015-12-02
