miRNAseq of extracellular vesicles from immortalized dental pulp mesenchymal stem cells from three different biopsies. miRNAseq of extracellular vesicles from immortalized dental pulp mesenchymal stem cells from three different biopsies

Extracellular vesicles (EVs) derived from three different biopsies of dental pulp mesenchymal stem cells (MSC-EVs) were isolated and characterized by different omic analysis. The goal was to characterized the EVs cargo to study their implication in an animal model of chronic cardiac inflammation Overall design: MSC-EVs were isolated form immortalized dental pulp MSC by tangential flow-filtration followed by size exclusion chromatography (TFF-SEC). The MSC-EVs were injected intramyocardially in a model of acute myocardial infarction (AMI) in nude rats, and their therapeutic effect was assessed 7 and 21 days post-infarction