Single-nucleus RNA-Sequencing (sci-RNA-seq3) of whole livers from 48 Four Core Genotype (FCG) mice

This repository contains single-nucleus gene expression profiling from 48 mouse livers of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes are on a C57BL6 genetic background. From all four genotypes, whole livers were dissected and flash frozen, followed by nuclear extraction and fixation for sci-RNA-seq3.本数据集仓库包含48只经四核心基因型杂交（Four Core Genotypes cross）的小鼠肝脏的单细胞核基因表达谱数据。研究通过将同时敲除Y染色体上性别决定因子Sry（sex-determining factor）、且3号染色体上转入Sry转基因的小鼠进行杂交，可获得四种性腺（睾丸或卵巢）与染色体核型（XX或XY）的组合，分别为：XYSry-Chr3Sry+（性腺与染色体均为雄性）、XYSry-（性腺为雌性、染色体为雄性）、XX（性腺与染色体均为雌性）、XXChr3Sry+（性腺为雄性、染色体为雌性）。所有转基因小鼠均以C57BL6为遗传背景。针对上述四种基因型的小鼠，研究人员均剥离其全肝组织并进行快速冷冻，随后开展细胞核提取与固定操作，用于sci-RNA-seq3测序。