Inducible CCR2+ nonclassical monocytes mediate the regression of cancer metastasis

Classical monocytes (CMs) can be converted into conventional dominant Nr4a1-dependent nonclassical monocytes (N-NCMs). Upon activation of Nod2 signaling, CM also can be converted into noncanonical Nod2-dependent inducible NCMs (I-NCMs). The transcriptional profiles and typical markers specific for those two NCM subsets yet to be determined. To transcriptionally characterize N-NCM and I-NCM, we performed transcriptional profiling analysis using RNAseq data obtained from the flow-sorted NCM subsets. Through the comprehensive RNAseq analysis, we identified typical transcriptional markers specific to N-NCM and I-NCM. By virtue of those specific markers, we identified the potential I-NCM populations in multiple cellular settings. To purely collect N-NCM and I-NCM, we sorted the blood NCMs from MDP-treated Nod2KO mice and Nr4a1KO mice, respectively. The flow-sorted NCMs were used for bulk RNA sequencing.