<b>A Dataset of NSC34 Motor Neuron Coding and Non-coding Transcriptome Following TDP-43 Knockdown and Mutant TDP-43 M337V Expression</b>

Mutations in the Transactive Response DNA-binding Protein 43 (TDP-43) and its loss of function in the nucleus are linked to several neurodegenerative diseases, including ALS, FTLD, and AD. TDP-43 is essential for RNA processing; therefore, TDP-43 proteinopathy can disrupt the cellular transcriptome. To investigate this, we used RNA interference to knock down TDP-43, and overexpressed the ALS-associated TDP-43 M337V mutation in mouse motor neurons cell line NSC34.RNA-seq (Illumina, total transcriptome, single end reads, 150bp, 8 replicates per sample) was conducted on all the samples: TDP-43 Knock-Down (KD), Control (NS), Overexpression of mutated TDP-43 (MVT), and Over-expression of wildtype TDP-43 (WTT). The gene expression results (raw counts, and TPMs) are presented in this repository, merged for all samples in the same flat text file.<b>Raw reads and protocol details can be found in ArrayExpress with Accession:</b> E-MTAB-13738<br><b>Study type:</b> Bulk RNA-seq of coding RNA<br><b>Organism:</b> Mus musculus<br><b>Note:</b> This dataset is a companion of the figshare dataset: https://doi.org/10.6084/m9.figshare.29256263<b>Raw data citation:</b><br>Ismail Gbadamosi, Sandra Binias, Bartłomiej Gielniewski, Ramiro Magno, Isabel Duarte and Ali Jawaid. "Comprehensive Transcriptomic Analysis of NSC34 Motor Neurons following TDP43 Modulation: Knockdown vs. Control and Wild Type vs. TDP43 M337V Mutation." <i>BioStudies</i>, E-MTAB-13738, 2025, https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-13738.<br>