Time and spatially-resolved Fourier-Transform Infrared (FTIR) Spectromicroscopy of cellulose in buffered reactions with Trichoderma reesei Cel7A

Synchrotron radiation Fourier transform infrared (SR-FTIR) spectra of cellulose undergoing hydrolysis by Trichoderma reesei Cel7A in buffer were collected. IR spectra were recorded at 64 locations every ~ 10 min in an 8x8 grid within a 70 x 70 μm sample area. , Enzyme

Trichoderma reesei Cel7A purified from Celluclast 1.5L (Sigma C2730)

Cellulose

Algal cellulose fibrils from Cladophora aegagropila

Reaction conditions

37oC
50 mM sodium acetate buffer, pH 5

 
Synchrotron Radiation Fourier Transform Infrared (SR-FTIR) spectromicroscopy of cellulose undergoing enzymatic hydrolysis
Experimental samples for monitoring cellulose hydrolysis by SR-FTIR were prepared by depositing and air-drying 60 mL of algal cellulose (0.025mg/mL) fibrils onto the SiNx membrane in an open-channel microfluidics (OMM) device, followed by an infiltration of 20 mL of Cel7A (0.5 µmole/g) through the cellulose. The enzyme and substrate was equilibrated in air at ambient conditions (~20oC) for ~ 40 minutes at room temperature, then mounted onto the FTIR microscope for optical alignment and location of regions of interest (ROI). The experiment was initiated by flowing 1 mM sodium acetate buffer at 0.3 mL/min into the fluid distribution channel connected to the bottom m..., , # Time and spatially-resolved Fourier-Transform Infrared (FTIR) Spectromicroscopy of cellulose in buffered reactions with Trichoderma reesei Cel7A

This dataset contains FTIR spectral data of cellulose undergoing hydrolysis by *Trichoderma reesei* Cel7A in sodium acetate buffer at pH 5. Synchrotron radiation infrared imaging was conducted on a ~50x50 micrometer area, within which spectral data were collected at 64 locations in an 8x8 grid, every ~9.5 minutes over ~15 hours. Spectral intensities in the wavelength range of 649 - 4000 1/cm were recorded.

## Description of the Data and file structure

FTIR data were collected at 64 different locations in an 8x8 grid. Data filenames corresponds to location in the 8x8 grid where spectra were obtained ('e.g. '1.csv' contain spectra recorded at point 1):

| **57.csv** | **58.csv** | **59.csv** | **60.csv** | **61.csv** | **62.csv** | **63.csv** | **64.csv** |
| :--------- | :--------- | :--------- | :--------- | :--------- | :--------- | :----...