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Platelet content of surface receptors.

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Figshare2015-12-02 更新2026-04-29 收录
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Platelets rich plasma was incubated with anti-GPIbα-DyLight649 antibody (Xia.G5), anti-αIIbβ3-FITC (Leo.F2), FITC-labeled rat anti-mouse GPVI and FITC-labeled rat anti-mouse integrin alpha2 chain (CD49b) or control isotype-matched mouse IgGs for 30 minutes at room temperature (Emfret Analytics, Eibelstadt, Germany). The samples were then washed, fixed with 1% PFA, and analyzed in a cytometer EPICS XL FACS. The results are means ±SEM of 4 different observations in duplicate. Not significant differences were detected between control and Podxl-null platelets.

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2015-12-02
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