Sequencing of RNA from Sctup1, Cgtup1, and Cgtup11 deletion strains

TUP1 is a well characterized general repressor of transcription in Saccharomyces cerevisiae and Candida albicans and is observed as a single copy gene. We observe that most species that experienced a whole genome duplication outside of the Saccharomyces genus have two copies of TUP1 in the Saccharomycotina yeast clade. We focus on Candida glabrata and demonstrate that the uncharacterized TUP1 homolog, CgTUP11, is most like the S. cerevisiae TUP1 gene through phenotypic assays and RNA-seq. Present in this RNA sequencing dataset are: Cgtup1, Cgtup11, and Cgtup1tup11 deletion strains compared to wild-type, and Sctup1 with either CgTUP1, CgTUP11, or ScTUP1 provided on a plasmid compared to an empty vector.