Hi-CoP, a simple and robust assay for detecting interactions of regulatory regions

First we developed a new, simple, and robust assay called CoP (Column Purified chromatin) for profiling of accessible regions by directly purifying fragmentized crosslinked chromatin with DNA purification column. The CoP chromatin regions by CoP-seq are consistent with the accessible regions detected by ATAC-seq and FARIE-seq. Then we integrated CoP-seq and Hi-C technique (Hi-CoP) for interactions of accessible chromatin regions which represent active cis-regulatory elements in cells. Our data showed that Hi-CoP assay can robustly detect interactions of regulatory regions. Overall design: Open chromatin were isolated with DNA purification column in K562 cell line and 4-12 weeks C57BL/6 WT mice thymocytes integrated Hi-C technique (Hi-CoP)or not.