Paratya australiensis protein-coding transcriptome
收藏Research Data Australia2024-12-14 收录
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https://researchdata.edu.au/paratya-australiensis-protein-coding-transcriptome/758201
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资源简介:
A collection of DNA and deduced protein sequences corresponding to the protein-coding transcriptome of adult specimens of the freshwater atyid shrimp Paratya australiensis. The sequences are derived from specimens from a laboratory-based experiment involving the exposure of shrimp to drain water from a site affected by acidification due to oxidation and re-wetting of acid sulphate soils. Please see associated publication in BMC Genomics (Bain et al., De novo assembly and analysis of changes in the protein-coding transcriptome of the freshwater shrimp Paratya australiensis (Decapoda: Atyidae) in response to acid sulfate drainage water, BMC Genomics, accepted manuscript).\nLineage: Total RNA was isolated from control specimens and specimens exposed to acid drainage water and mRNA was sequenced by a service provider using an Illumina HiSeq2000 instrument. Library preparation was strand-specific and sequences were generated using paired-end mode with 100 cycles. Putative transcripts were assembled in a de novo fashion (without a reference genome) using Trinity software (v. 2.0.6). Protein sequences were deduced using Transdecoder software (v. 2.0.1). Likely descriptions and functions were assigned using Blast2GO command-line v. 1.0.2.
提供机构:
Commonwealth Scientific and Industrial Research Organisation



