Whole genome shotgun sequencing of EMS treated Hordeum vulgare cv. Bowman and BW230 individuals to study cross over distribution.

In our experiment we treated the Hordeum vulgare cv. Bowman and the NIL BW230 with 25mM EMS to introduce mutations in the genome. We used 15x whole genome shotgun sequencing of one M2 plant respectively to establish high confidence heterozygous positions in the genome induced by EMS and used those as a reference. We did whole genome shotgun sequencing on 25 progeny M3 individuals. Using the M2 heterozygous reference files we rescored those positions in the M3 individuals to establish changes from heterozygous to homozygous SNPs. Phase changes were used to score the number of cross overs, their distribution in the genome and differences in frequency between Bowman and BW230.