Binding of Pi to active site nucleophile mutants of AP.
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aThe observed dissociation constants were determined at pH 8.0 in 100 mM NaMOPS, 100 mM NaCl, 1 mM MgCl2, and 100 µM ZnCl2 at 4°C.bK is the ratio of the observed Pi dissociation constant in the presence of Ser102 compared to the dissociation constant in the absence of Ser102: Kd(Ser102)/Kd(Gly or Ala102). Numbers greater than 1 represent stronger binding.
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2015-12-02



