High-throughput RNA sequencing analysis of mouse pre-pro-B cells and pro-B cells from wild type and Lactoferrin knockout mouse. High-throughput RNA sequencing analysis of mouse pre-pro-B cells and pro-B cells from wild type and Lactoferrin knockout mouse

Purpose: The goal of this study is using RNA sequencing analysis to evaluate the role lactoferrin in the early development of B cells in mouse Methods: The pre-pro-B cells and pro-B cells were isolated from the wild type and lactoferrin knockout mice (n=3 for each group) by flow cytometry. Then the cell RNA was sequenced by the NovaSeq 6000 high-throughput sequencing service (Jingneng, Shanghai, China). Data were extracted and normalized according to the manufacturer’s standard protocol. Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. Conclusions: Our study describes the mRNA changes of pre-pro-B and pro-B cells from wild type and lactoferrin knockout mice Overall design: The pre-pro-B and pro-B cells were isolated from the wild type and lactoferrin mice, and cellular RNA was used for RNA-sequencing to show the gene expression profile change induced by lactoferrin deficiency