Effects of BPA, BPF and BPS treatments on mESC EB-based global and neural differentiations.

Purpose: the goal of this study is to determine the changes in gene expression during EB differentiation after BPA, BPF and BPS treatments, as indications of potential developmental toxicity. Methods: Transcriptomics profiling for EB samples treated with 100 nM BPA, BPF, BPS or DMSO control at different time points were generated by RNA-seq, using a BGISEQ-500 platform. Reads were filtered and aligned to the reference genome with Bowtie2. Results: BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, analogous gene categories were differentially regulated by the three chemicals, at each time point, particularly the ones involved in cell-matrix and cell-cell adhesions, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Overall design: Samples treated with 100 nM BPA, BPF, BPS or DMSO were collected at differentiation days 0, 4, 6, 9, 12 and 20 for global EB differentiation, and at days 0, 4, 8 and 10 during EB/NPC differentiation.