Alcohol-associated hepatitis induces hepatocyte senescence which is reduced following disease resolution I

Alcohol-associated hepatitis (AH) is an acute form of alcohol-related liver disease (ArLD) with high mortality rate. AH is histologically characterised by cellular processes including steatosis, inflammation and cell death. Apoptosis is the most studied form of cell death in AH; however, the role of cellular senescence, another response to cellular injury, in AH is unknown. Here we aim to explore the mechanisms of ArLD pathophysiology and define the role of senescence in AH. VL-17A cells (a modified human hepatocellular HepG2 cell line which overexpress CYP2E1 and ADH1) were treated with 100mM ethanol for 48 hours and compared to untreated VL-17A cells using RNA-seq. Media was replaced every 24 hours with fresh media and ethanol to minimize effects of evaporation. 4x samples per condition.