Effect of 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq data

To investigate the effect of increasing 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq, we labeled 3 different cell lines with increasing concentrations of 4sU. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples. Increasing labeling concentrations (up to 800 µM) in U2OS, HCT116 and HFF-TerT cells for up to 2 hours in 2 replicates.