Inactivation of urod in zebrafish blood cells

We wanted to evaluate the proportion of red blood cells in pcmlc2:GFP, zU6:gRNA urod, gata1:zCas9 embryos that show red fluorescence. Conclusion: We detect red fluorescent cells in the blood of pcmlc2:GFP, zU6:gRNA urod, gata1:zCas9 zebrafish embryos (36 hpf and 48hpf). Notes: However, a large percentage of these cells appear to be dead/dying compared to WT siblings, indicating that they are particularly fragile and that we may have an underestimation of the proportion of urod mutant cells at a given timepoint. Compared injected embryos to WT sibling controls and carefully controlled for auto-fluorescence during FACS.