Genome annotation file for a draft genome assembly for Nucella lapillus

A male specimen of wild Nucella lapillus, measuring approximately 1.5–3 cm in length, was collected from a rocky shore (mid-upper shore) at low tide from near the quay at Portnahaven, Isle of Islay, Argyll and Bute, Scotland (National Grid Reference NR 16614 51966) on 26th June 2023. Genomic DNA was extracted from the non-shell tissue of the specimen, and sequenced using PacBio HIFI and Oxford Nanopore technolgies (ONT) long read sequencing platforms. The genome assembly was derived from 40.6 Gb of PacBio HiFi reads (read N50, 11291; N90, 9246), and 61.1 Gb of ONT data (read N50, 3643; N90, 1546). Annotation of protein-coding genes in the cleaned and masked genome assembly of Nucella lapillus was performed using GALBA v1.0.11, an automated pipeline that uses proteins from a closely related species to assist in the training of gene prediction using AUGUSTUS. Proteins from Rapana venosa were provided for this purpose, and the miniprot option was used to perform the protein-to-genome alignments. Functional annotation of predicted protein-coding genes was performed using eggNOG-mapper v2.1.12, and additionally annotated with best hit BLAST results (v2.16.0) against the proteomes of the following marine gastropod species: Rapana venosa, Littorina. saxatilis, Pomocea canaliculata, Stramonita haemastoma and Haliotis rufescens.