Comparative densitometric analysis of pH and calcium-dependent Yops secretion in Y. pseudotuberculosis.

To compare and quantify the Yop secretion efficiency in wild-type Y. pseudotuberculosis under different conditions, Coomassie stained Yop secretion profiles were subjected to densitometric analysis with Multi Gauge software (Fuji Film). The protein YopJ (boxed in Figure 4 and Figure 5E) was selected for quantitative analysis. Growth kinetics in media with different pH’s (Figure S4B) showed attenuation of bacterial growth directly linked to the observed Yop secretion efficiency.asecretion efficiency was set to 100%;bsecretion efficiency at pH 7.5 was set to 100%.

为比较和量化野生型耶尔森氏菌在多种条件下的Yop分泌效率，采用Coomassie染色法对Yop分泌谱进行密度扫描分析，分析软件为Multi Gauge（富士胶片）。在图4和图5E中框定的蛋白质YopJ被选为定量分析对象。不同pH值培养基中的生长动力学（图S4B）显示，细菌生长的衰减与观察到的Yop分泌效率直接相关。将分泌效率设定为100%；pH值为7.5时的分泌效率设定为100%。