Proteomic analysis of human serum from patients with Chronic kidney disease

We applied two-dimensional fluorescence difference gel electrophoresis (2D DIGE) approach to separate depleted serum proteins in CKD and control serum. High abundance proteins were deleted to improve visualization of protein spots. After differentially expressed proteins were identified, these proteins were quantitated with multiple reaction monitoring (MRM) in the serum samples of patients (n=26) and control groups (n=10).