Structural analysis of protein machineries using single particle cryo-EM

Structures of large macromolecular machineries were for long time not studied at atomic level as they were often too large for X-ray crystallography and as electron microscopy...长期以来，大型大分子分子机器的结构始终未能在原子分辨率水平上得到解析，究其原因是这类复合物往往体积过大，无法满足X射线晶体学（X-ray crystallography）的实验条件，同时电子显微镜（electron microscopy）……