Hippo signaling instructs ectopic but not normal organ growth [Liver YapHyper]

We conditionally activated Yap in hepatocytes of adult mice by two different methods: we deleted the Lats1/2 kinases via injecting AAV8-Cre into Lats1/2 double floxed mice (Lats1/2 KO) and we overexpressed YAP via doxycycline feeding of mice that expressed the tetracycline-activated transactivator (rtTA) under the hepatocyte-specific ApoE promoter and human YAP from a rtTA-responsive TetO promoter (Apo>YAP). Differential Gene Expression Analysis of Yap hyperactivation in adult hepatocytes including samples of purified hepatocytes from livers in the following condtions: Apo>Yap (ApoYap Dox) and untreated control (ApoYap ctrl); Lats1/2 KO and untreated control (Lats1/2 flox ctrl); BL/6 controls for the side effects of Doxycyline and AAV-Cre (BL/6 ctrl, BL/6 Dox, BL/6 AAV-Cre.