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Comparison of PCR versus PCR-free DNA library preparation for characterising the human faecal virome

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NIAID Data Ecosystem2026-05-01 收录
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The raw sequencing reads were derived from the human faecal virome. Total VLP DNA isolated from faecal samples provided by three donors was subjected to whole-genome shotgun metagenomic sequencing using Illumina HiSeq X Ten platform. In this study, we compared the use of PCR and PCR-free methods for sequence library construction to assess the impact of PCR amplification bias on the human faecal virome.

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2023-10-13
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