The data underlying Figs 4 and S6.

(A) SKI-II eradicated infected cells from cultures infected with CTL2. Bacterial inclusions were detected by microscopy at 34 hpi. Data are displayed in Fig 4A. (B) MYR and LCS, but not GW4869, reduced inclusion count after infection with the cpoS mutant. Bacterial inclusions were detected by microscopy at 25.5 hpi. Data are displayed in Fig 4C. (C) Differential susceptibility of CTL2 and CTL2-cpoS::cat to SPT inhibition. GFP fluorescence, as a measure of bacterial growth, was detected at 25.5 hpi. Data are displayed in Fig 4E. (D) Depletion of SPTLC1 or deficiency in KDSR partially restored inclusion numbers after infection with the cpoS mutant. Bacterial inclusions were detected by microscopy at 24 hpi. Data are displayed in Fig 4F. (E) A blockade in membrane trafficking sensitized CTL2 to the growth-inhibitory action of MYR. Bacterial inclusions were detected by microscopy at 20 hpi. Data are displayed in S6A Fig. (F) Normalized read counts for CERT-targeting sgRNAs in the CRISPR screen. Data are displayed in S6B Fig. (G) Depletion of CERT partially protected cells from cpoS mutant-induced death. Host cell viability based on resorufin fluorescence measured 25.5 hrs post infection of the indicated cell lines with the indicated bacterial strains. Data are displayed in S6D Fig. (H) Depletion of CERT did not significantly modify inclusion numbers after infection with the cpoS mutant. Bacterial inclusions were detected by microscopy at 24 hpi. Data are displayed in S6E Fig. (XLSX)