RNA-seq analysis further corroborated the reversal effect of asp on AOA-enhanced ISG expression in L929 cells

To assess the functional significance of asp in the cGAS-STING pathway, these two metabolites were individually added along with AOA to the culture medium, followed by HT-DNA stimulation for 6 hours. Interestingly, asp fully reversed the amplification effect of A on STING-mediated immune response Overall design: L929 cells were treated with 0.5 mM A for 1h followed by HT-DNA stimulation for 6h in the absence or presence of 20 mM asp, and then cells were harvested for qPCR analysis of IFN response gene expression