Summary of specificities of several characterised GH27 enzymes, and motivation for the mutations of CpArap27.

Kinetic parameters for several GH27 enzymes, with different substrate specificities and different amino acids in a key active site position, are compared. Kinetic parameters for all forms of CpArap27 are given in Table 1. The Ara:Gal ratios were determined by dividing kcat / Km values and normalising the smaller value to 1.a The Ara:Gal ratio of each enzyme or enzyme variant compares kcat / Km for hydrolysis of pNP-β-l-Arap and pNP-α-d-Galp respectively.Summary of specificities of several characterised GH27 enzymes, and motivation for the mutations of CpArap27.