WT MSCs displayed a different transcriptional profile than KO MSCs.
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https://www.ncbi.nlm.nih.gov/sra/SRP405865
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To explore the molecular basis of the therapeutic function of MSCs, we performed RNA-sequencing of LPS-treated MSCs.Transcriptome analysis identified the upregulation of 673 genes and downregulation of 831 genes, and plot the top 100 differentially expressed genes into a heatmap.KEGG pathway enrichment analysis indicated that energy metabolism pathways were highly enriched in the cell cycle. Overall design: WT and KO MSCs were stimulated with/without 3 µg/mL LPS for 24?h.Total RNA was extracted and purified using TRIzol reagent (Invitrogen, USA) according to the manufacturer's protocol.The library was sequenced with Illumina Novaseq⢠6000 by 2Ã150bp paired-end sequencing (PE150) (LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol. T
创建时间：
2024-11-07
