Gene expression in hTERT-RPE1 cells with overexpression of MFRP. Homo sapiens

Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion. Overall design: RNA was prepared from hTERT-RPE1 cells overexpressing GFP-MFRP and control cells. Both conditions were done in triplicate. Affymetrix GeneChip Human Genome U133Plus2.0 arrays were used to interrogate changes in gene expression. Image data were quantified with Affymetrix Expression Console Software and normalized with Robust Multichip Analysis. Reference: Joseph Fogerty. 2013. "A novel mouse mutant resembling age-related macular degeneration". A PhD Dissertation submitted to the Graduate School of Biomedical Sciences of the Medical College of Wisconsin, Milwaukee (April 2013).