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The CosMx SMI data used in this study were obtained from the public repository Gene Expression Omnibus (GEO) under accession number GSE289194. Data preprocessing, including quality control, normalization, and clustering based on the Seurat SCT pipeline, as well as initial cell type annotation, were performed using the script 01_processing.R. Subcluster annotation of B cells and T cells was conducted using 02_BCell_TCell_annotation.R. The spatial distance analysis between NK cells and tumor cells was performed using 03_NK_distance_analysis.R.