Spectral flow cytometric tracking of mitochondria transfer in adipose tissue by age and diet

Unmixed fcs files for the stromal vascular fraction of epididymal (eWAT), inguinal (iWAT), and brown adipose tissue (BAT) in mtD2F/+AdipoqCre+/- and different ages or diets. 

For a breakdown of samples and code, please see: https://github.com/ncborcherding/mtD2. The full github repository at time of manuscript acceptance is also in the Zenodo repository under mtD2_GitHubRepo.zip.

Antibody Panel: 

Laser

Channel

Antigen

Fluorophore

Final Dilution Factor

Vendor

Clone

Catalog Number

Violet

V1

SiglecF

BV 421

1:400

BD Biosciences

E50-2440

562681

V2

CD44

Super Bright 436

1:300

eBioscience

IM7

62-044-180

V3

CD11b

Pacific Blue

1:300

BioLegend

M1/70

101224

V5

IL-33R⍺-biotin

n/a

1:300

BioLegend

DIH9

145308

V5

Streptavidin (2°)

BV 480

1:300

BD Horizon

n/a

564876

V7

MHC-II (I-A/I-E)

BV 510

1:300

BioLegend

M5/114.15.2

107636

V8

Ly6C

BV 570

1:400

BioLegend

HK1.4

128030

V10

KLRG1

BV 605

1:300

BioLegend

2F1/KLRG1

138419

V11

F4/80

BV 650

1:300

BioLegend

BM8

123149

V13

Ly6G

BV 711

1:300

BioLegend

1A8

127643

V14

CD3

BV 750

1:300

BioLegend

17A2

100249

V15

CD206

BV 785

1:200

BioLegend

C068C2

141729

Blue

B1

mtDendra2

n/a

n/a

n/a

n/a

n/a

B2

TCRb

AF 488

1:300

BioLegend

H57-597

109215

B3

CD8

Spark Blue 550

1:300

BioLegend

53-6.7

100780

B8

CD45

PerCP

1:200

BioLegend

30-F11

103130

B9

CD71

PerCP-Cy5.5

1:200

BioLegend

RI7217

113816

B10

NKp46

PerCP-eFluor710

1:200

eBioscience

29A1.4

46-3351-82

Green

YG1

TCRgd

PE

1:300

BioLegend

GL3

118108

YG3

CD64

PE/Dazzle 594

1:300

BioLegend

X54-5/7.1

139320

YG4

CD4

PE/Fire 640

1:300

BioLegend

GK1.5

100482

YG5

CD62L

PE-Cyanine5

1:300

BioLegend

MEL-14

104410

YG7

CD11c

PE-Cyanine5.5

1:300

eBioscience

N418

35-0114-82

YG9

CD25

PE/Cyanine7

1:200

BioLegend

PC61

102016

Red

R1

CD9

APC

1:300

BioLegend

MZ3

124812

R2

CD127

AF 647

1:200

BioLegend

A7R34

135020

R3

CD19

Spark NIR 550

1:300

BioLegend

6D5

115568

R4

CD90.2

AF 700

1:300

BioLegend

30-H12

105320

R5

CD5

BUV 737

1:300

BD Horizon

53-7-3

612809

R6

Viability dye

Zombie NIR

1:1,000

BioLegend

n/a

423106

R7

NK1.1

APC/Fire 750

1:300

BioLegend

PK136

108752

R8

B220

APC/Fire 810

1:300

BioLegend

RA3-6B2

103278