Nanopore sequencing of UNC13A from SHSY5Y and patient RNA. Nanopore sequencing of UNC13A from SHSY5Y and patient RNA

UNC13A contains a novel cryptic exon which is expressed upon TDP-43 knockdown. However, it also features TDP-43 regulated intron retention of a downstream intron. To investigate the correlation of these two events, we performed Nanopore sequencing of amplicons from SHSY5Y cells with inducible TDP-43 knockdown, and FTD patient RNA samples